Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L
Potassium Phosphate Buffer (0 2 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Vector Laboratories Casein Solution 10X 250 ml
Casein Solution is a general blocking agent for immunohistochemistry assays, nucleic acid and protein blotting, and other applications. In this product, it is supplied as a 10x concentrate.
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 5mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Research Products International Corp RPI TRIS-CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
This product is shipped with one 500 ml Anode bottle and one 500 ml Cathode bottle
10X Anode Formulation:
TRIS: 0.6 M
CAPS: 0.4M
10X Cathode Formulation:
TRIS: 0.6 M
CAPS: 0.4 M
SDS: 1%
Research Products International Corp ADA, 25 G
- Substance Name: [N-(Carbamoylmethyl) iminodiacetic acid)] [N-(2-Aceta-mido)iminodiacetic acid]
- CAS Number: 26239-55-4
- Molecular Formula: C6H10N2O5
- Molecular Weight: 190.2
Sigma Aldrich Fine Chemicals Biosciences BES 99.0 titration250G
BES (N N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES a sulfonic acid-containing cross-linking agent induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
Teknova 1M Tris-HCl, pH 9.2. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova Assay Diluent. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 2 pack.
2M Tris,3M Sodium Chloride,1.0% Tween-20
Teknova 4M Tris-HCl, pH 7.5. 250mL, RNase/DNase Tested. Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Research Products International Corp TRIS-Glycine-SDS Buffer, 10X Powder, 184 Grams of Powder, Makes 1 Liter of Solution
Composition:
TRIS (Hydroxymethyl) Aminomethane: < 20.0%
SDS: < 6.0%
Electron Microscopy Sciences Tris Maleate Buffer 0.2M, pH range 6.4-8.0 250 ML
Please specify pH when ordering.
pH: 6.4-8.0
Electron Microscopy Sciences Tris (Hydroxymethyl) Aminomethane Free Base, Reagent 100 GR
CAS #77-86-1 C₄H₁₁NO₃ F.W. 121.14
Assay: >99.9%
White crystalline powder. A 40% (w/v) aqueous solution. Hydroxymethyl Aminomethane.
Grade: Reagent
Electron Microscopy Sciences Tris (Hydroxymethyl) Aminomethane Free Base, Reagent 500 GR
CAS #77-86-1 C₄H₁₁NO₃ F.W. 121.14
Assay: >99.9%
White crystalline powder. A 40% (w/v) aqueous solution. Hydroxymethyl Aminomethane.
Grade: Reagent