Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl, pH 8.0. 500mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
PHOSPHATE Phosphate buffered saline
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Chemscene ChemScene | 2,2-Difluorocyclopropan-1-amine hydrochloride | 250MG | CS-W000385 | 0.98 | 105614-25-3| MFCD07777150 | 129.54
ChemScene | 2,2-Difluorocyclopropan-1-amine hydrochloride | 250MG | CS-W000385 | 0.98 | 105614-25-3| MFCD07777150 | 129.54
Research Products International Corp Blot Stripping Buffer 4X Solution, 250 ML
Product Formulation: TRIS-HCl: 250 mM; SDS: 8%; pH: 6.8. Add mercaptoethanol according to protocol.
Instructions :
1. Dilute 4X Stripping Bufffer ( 1part Stripping Buffer: 3 parts distilled water) and add beta-mercaptoethanol (0.6%).
2. Wash the wet membrane in the stripping buffer for 5 minutes.
3. Incubate the membrane in stripping buffer for 30 minutes at 65 degrees Celius while agitating.
STEMCELL Technologies D-PBS (Without Ca++ and Mg++), Size: 500 mL
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating, or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions.
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 100g
Trizma base | Purity: 99.9% | Mol Wt: 121.41 | 77-86-1 | MFCD00004679 | 100g
Sigma Aldrich Fine Chemicals Biosciences BIS TRIS propane BioXtra 9100G
BIS TRIS propane BioXtra 9100G
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer Bi1L
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This SOLUTIONon is effective under slightly basic conditions which keeps DNA deprotonated water-soluble and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
Sigma Aldrich Fine Chemicals Biosciences MES Hydrate | 1266615-59-1 | MFCD00149409 | 1kg
MES Hydrate | Purity: 99% | Mol Wt: 195.24 (anhydrous basis) | 1266615-59-1 | MFCD00149409 | 1kg